Semi-rational approach for converting a GH36 α-glycosidase into an α-transglycosidase
نویسندگان
چکیده
منابع مشابه
Semi-rational approach for converting a GH1 β-glycosidase into a β-transglycosidase.
A large number of retaining glycosidases catalyze both hydrolysis and transglycosylation reactions, but little is known about what determines the balance between these two activities (transglycosylation/hydrolysis ratio). We previously obtained by directed evolution the mutants F401S and N282T of Thermus thermophilus β-glycosidase (Ttβ-gly, glycoside hydrolase family 1 (GH1)), which display a h...
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ژورنال
عنوان ژورنال: Glycobiology
سال: 2014
ISSN: 1460-2423,0959-6658
DOI: 10.1093/glycob/cwu124